Coding

Part:BBa_K2372000:Design

Designed by: Marcel Chris Conrady   Group: iGEM17_Tuebingen   (2017-10-26)


Amide synthetase from Coumermycin A1 biosynthesis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1529
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 470
    Illegal NgoMIV site found at 700
    Illegal NgoMIV site found at 1022
    Illegal NgoMIV site found at 1129
    Illegal AgeI site found at 75
    Illegal AgeI site found at 122
    Illegal AgeI site found at 550
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 388
    Illegal BsaI.rc site found at 955
    Illegal BsaI.rc site found at 1073


Design Notes

This part has a high GC content.


Source

Streptomyces

References